| Expression pattern: |
UP |
| Associated gene: |
Rab10, cyclin D1, CDK4, Bcl-2, cleaved caspase-3 |
| Associated microRNA: |
miR-203 |
| Biological function: |
Promotes AML cell proliferation and cell cycle progression, inhibits apoptosis, and regulates proliferation- and apoptosis-related proteins. |
| Molecular mechanism: |
hsa_circ_100290 acts as a miR-203 sponge to regulate the miR-203/Rab10 axis in AML cells. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay |
| Clinical significance: |
The study suggests hsa_circ_100290 may provide novel insights into AML diagnosis and potential future therapies. |
| Description: |
hsa_circ_100290 is up-regulated in AML bone marrow samples and AML cell lines. It promotes AML cell proliferation and cell cycle progression while inhibiting apoptosis, at least partly by sponging miR-203 and regulating Rab10 and apoptosis/cell-cycle related proteins. |
| Confidence score: |
0.7591 |