| Expression pattern: |
UP |
| Associated gene: |
SH2B1, E-cadherin, Vimentin, Ki67 |
| Associated microRNA: |
miR-136 |
| Biological function: |
hsa_circ_0136666 promotes CRC cell proliferation, migration, invasion, cell cycle progression, EMT-mediated invasion, and tumor growth in vivo; knockdown suppresses these malignant phenotypes and arrests cells in G0/G1 phase. |
| Molecular mechanism: |
hsa_circ_0136666 functions as a ceRNA/molecular sponge for miR-136, thereby increasing SH2B1 expression and promoting CRC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); EMT (promotes) |
| Detected method: |
Q
S
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Luciferase Reporter Assay; RNA Pull-Down; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0136666 expression was associated with poor overall survival of patients with CRC, suggesting potential biomarker and therapeutic relevance. |
| Description: |
hsa_circ_0136666 is up-regulated in colorectal cancer tissues and cell lines and high expression predicts poorer overall survival. Functionally, it acts as an oncogenic circRNA that promotes proliferation, migration, invasion, EMT-related changes, cell cycle progression, and xenograft tumor growth. Mechanistically, it sponges miR-136 to increase SH2B1 expression, forming the hsa_circ_0136666/miR-136/SH2B1 regulatory axis. |
| Confidence score: |
0.8354 |