| Expression pattern: |
UP |
| Associated gene: |
AGO2, ZEB2 |
| Associated microRNA: |
miR-145 |
| Biological function: |
circNUP214 promotes PTC cell proliferation, migration, invasion, and tumorigenesis; circNUP214 knockdown inhibits these malignant phenotypes. |
| Molecular mechanism: |
circNUP214 acts as a ceRNA sponge for miR-145, thereby relieving miR-145-mediated repression of ZEB2 and promoting ZEB2 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Bioinformatics Analysis; Microarray; RT-qPCR; Clinical Sample Validation; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
circNUP214 is upregulated in clinical PTC specimens and can serve as a potential therapeutic target in PTC treatment. |
| Description: |
circNUP214 is upregulated in papillary thyroid cancer tissues and cell lines. It functions as an oncogenic cytoplasmic circRNA by sponging miR-145, thereby increasing ZEB2 mRNA and protein expression and promoting PTC cell proliferation, migration, invasion, and xenograft tumor growth. The study proposes the circNUP214/miR-145/ZEB2 axis as a potential therapeutic target in PTC. |
| Confidence score: |
0.8206 |