| Expression pattern: |
UP |
| Associated gene: |
RAC1, Ago2, Bax, Bcl-2, Ki67 |
| Associated microRNA: |
miR-939-5p |
| Biological function: |
Promotes CRC cell proliferation, migration, invasion, tube formation/angiogenesis, and in vivo tumor growth, while inhibiting apoptosis. |
| Molecular mechanism: |
circ_0058123 acts as a ceRNA/sponge for miR-939-5p to positively regulate RAC1 expression and promote CRC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); metastasis (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Clinical Sample Validation; Transfection; EdU Staining; Transwell Assay; Tube Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
circ_0058123 expression was closely related with TNM stage, and it may be a valuable biomarker for early diagnosis and prognosis of CRC. |
| Description: |
circ_0058123 is up-regulated in CRC tissues and cell lines and is associated with higher TNM stage. It promotes CRC progression by sponging miR-939-5p and increasing RAC1 expression, thereby enhancing proliferation, migration, invasion, angiogenesis/tube formation, and tumor growth while suppressing apoptosis. The study proposes circ_0058123 as a potential biomarker and therapeutic target for CRC. |
| Confidence score: |
0.8169 |