circRNA basic information
circBase ID: -
Name: hsa_circ_DNAH14
Synonym: circular RNA DNAH14 / circDNAH14
Host Gene: DNAH14
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

PLC/PRF/5; HepG2; SMMC7721; SMMC-7721
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: PTMA, AGO2
Associated microRNA: miR-508-3p
Biological function: circDNAH14 attenuates hypoxia-induced suppression of HCC malignant behaviors (proliferation, invasion, migration, EMT) and reduces apoptosis; circDNAH14 knockdown enhances hypoxia-induced tumor suppression in vivo.
Molecular mechanism: circDNAH14 acts as a ceRNA by binding miR-508-3p and regulating PTMA expression; interaction supported by luciferase reporter and AGO2-RIP.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

In a cobalt chloride-induced hypoxia model mimicking TACE, circDNAH14 is down-regulated in hypoxic HCC cells and functionally counteracts hypoxia-induced suppression of malignant phenotypes. It sponges miR-508-3p to maintain PTMA expression (circDNAH14/miR-508-3p/PTMA), thereby promoting proliferation/migration/invasion/EMT and reducing apoptosis; circDNAH14 knockdown enhances hypoxia-induced tumor growth inhibition in xenografts.
Confidence score:

0.6831
Other information
Title:

Circular RNA DNAH14 molecular mechanism in an experimental model of hepatocellular carcinoma treated with Cobalt chloride to mimic the hypoxia-like response of transcatheter arterial chemoembolization.

Journal: Scientific reports
Published: 2024
PubMed ID: 38263208
Study type:

biological research
Data availability: The data that support the findings of this study are available from corresponding author but restrictions apply to the availability of these data, which were used under license for the current study, and so are not publicly available. Data are however available from the authors upon reasonable request and with permission of corresponding author.
Code availability: -