| Expression pattern: |
UP |
| Associated gene: |
NOVA2 |
| Associated microRNA: |
hsa-miR-7-5p / miR-7 |
| Biological function: |
circ-U2AF1 acts as an oncogene in glioma, promoting glioma cell proliferation, migration, invasion, metastasis-like phenotypes and tumorigenesis, while inhibiting apoptosis and affecting cell cycle progression. |
| Molecular mechanism: |
circ-U2AF1 functions as a competing endogenous RNA that sponges hsa-miR-7-5p, thereby derepressing and upregulating NOVA2 to promote glioma malignancy. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (other); p53 signaling (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circ-U2AF1 expression was positively correlated with glioma grade and circ-U2AF1 was suggested as a prognostic biomarker and therapeutic target. |
| Description: |
circ-U2AF1 (hsa_circ_0061868) is upregulated in human glioma tissues and glioma cell lines and its expression positively correlates with glioma grade. Functionally, circ-U2AF1 promotes glioma cell proliferation, migration, invasion, metastasis-like behavior and xenograft tumor growth, while suppressing apoptosis. Mechanistically, it sponges hsa-miR-7-5p to derepress NOVA2, forming a circ-U2AF1/hsa-miR-7-5p/NOVA2 regulatory pathway with potential prognostic and therapeutic relevance. |
| Confidence score: |
0.705 |