circRNA basic information
circBase ID: hsa_circ_0135889
Name: hsa_circ_AGO2
Synonym: circRNA generated from AGO2 / circAGO2
Host Gene: AGO2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008315
MONDO name: prostate cancer
Disease details: prostate cancer
Disease DO ID:
 10283
Disease MeSH ID:
 D011471
Disease NCIt ID:
 C7378
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

gastric cancer tissues; colon cancer tissues; prostate cancer tissues; neuroblastoma tissues; adjacent normal tissues; ganglioneuroblastoma tissues; xenografts
Cell lines:

MKN-45; AGS; HCT-116; PC-3; HEK293T; HeLa; LoVo; SH-SY5Y; SK-N-SH
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HuR, human antigen R, AGO2-miRNA complexes, HIP-13
Associated microRNA: -
Biological function: CircAGO2 promotes cancer cell growth, invasion, migration, metastasis, xenograft tumor growth, angiogenesis-related CD31-positive intratumoral microvessels, and poor outcome.
Molecular mechanism: CircAGO2 physically interacts with and activates HuR, promotes HuR translocation from nucleus to cytoplasm and enrichment on target-gene 3'-UTRs, decreases AGO2 binding, and represses AGO2/miRNA-mediated gene silencing, thereby increasing expression and stability of cancer progression-related target genes.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); angiogenesis (promotes); mRNA stability (promotes); other pathway/process (promotes)
Detected method:
Q
B
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Co-IP; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Actinomycin D / DRB Stability Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; Survival Analysis; Bioinformatics Analysis; RNA-seq
Clinical significance:

CircAGO2 is highly expressed in gastric cancer tissues with metastasis, and high circAGO2 expression is associated with worse overall survival and poor outcome.
Description:

CircAGO2 is a human AGO2-derived intronic circRNA that is up-regulated in gastric cancer, colon cancer, prostate cancer, and neuroblastoma. It functions as an oncogenic circRNA by binding and activating HuR, thereby reducing AGO2/miRNA-mediated gene silencing of cancer progression-related target genes and promoting tumor growth, invasion, metastasis, and poor prognosis.
Confidence score:

0.898
Other information
Title:

Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes.

Journal: Cell death and differentiation
Published: 2019
PubMed ID: 30341421
Study type:

combined biological and clinical study
Data availability: GSE114055; circBase; RBPDB; BioGRID; CLIPdb; Kaplan-Meier Plotter
Code availability: -