| Expression pattern: |
UP |
| Associated gene: |
YY1, Ago2 |
| Associated microRNA: |
miR-204-5p |
| Biological function: |
Promotes proliferation, invasion/migration and inflammatory response, and inhibits apoptosis in RA-FLSs (circ_0083964 knockdown shows opposite effects). |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-204-5p to regulate YY1 expression (circ_0083964/miR-204-5p/YY1 feedback loop). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); inflammation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; ELISA; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0083964 is up-regulated in RA synovial tissues and RA-FLSs and promotes RA-FLS tumorlike behaviors (viability/proliferation, invasion/migration) and inflammatory cytokine release while suppressing apoptosis. Mechanistically, it sponges miR-204-5p to relieve repression of YY1, forming a circ_0083964/miR-204-5p/YY1 regulatory axis. |
| Confidence score: |
0.8119 |