| Expression pattern: |
UP |
| Associated gene: |
hnRNPM, ribosomal protein L36, ribosomal protein L35A, ribosomal protein S15A, PGK1, S100A9 |
| Associated microRNA: |
- |
| Biological function: |
Promotes colorectal cancer progression, particularly migration; knockdown attenuates tumor growth in vivo. |
| Molecular mechanism: |
circ_0004662 acts as a non-coding circRNA that binds the RNA-binding protein hnRNPM; hnRNPM interference decreases circ_0004662 expression and affects CRC progression. |
| Biological pathway or process: |
migration (promotes); proliferation (promotes); metastasis (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
Potential target for treating CRC. |
| Description: |
circ_0004662 is up-regulated in colorectal cancer tissues and cell lines. Functionally, it promotes CRC malignant behavior, especially migration, and its knockdown suppresses tumor growth in a xenograft model. Mechanistically, circ_0004662 acts as a non-coding circRNA that binds the RBP hnRNPM, and hnRNPM depletion reduces circ_0004662 levels, supporting a circ_0004662-hnRNPM regulatory interaction in CRC progression. |
| Confidence score: |
0.8695 |