| Expression pattern: |
DN |
| Associated gene: |
RBM10, SF3B1 |
| Associated microRNA: |
- |
| Biological function: |
Tumor-suppressive: decreases colony formation and cell viability; rescues RBM10-loss tumorigenic phenotype; reduces xenograft tumor weight. |
| Molecular mechanism: |
RBM10 directly binds intronic flanking regions to modulate exon skipping and circularization; RBM10-SF3B1 axis regulates circHIPK3 formation; SF3B1 is epistatic to RBM10. |
| Biological pathway or process: |
proliferation (inhibits); cell cycle (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; CLIP; Transfection; Colony Formation Assay; CCK8; In Vivo Animal Model; Co-IP; Bioinformatics Analysis |
| Clinical significance: |
Downregulated in LUAD tumors (including RBM10-mutant tumors) and correlated with RBM10 expression; highlighted as a promising biomarker and potential therapeutic target in RBM10-deficient LUAD. |
| Description: |
circHIPK3 is an RBM10-dependent circRNA that is down-regulated in LUAD, especially in RBM10-mutant tumors. Functionally, increasing circHIPK3 suppresses LUAD cell growth and reduces xenograft tumor burden, consistent with a tumor-suppressor role linked to RBM10-mediated splicing/circularization control involving SF3B1. |
| Confidence score: |
0.7884 |