| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-124 |
| Biological function: |
circHIPK3 promotes diabetic neuropathic pain and neuroinflammation; silencing circHIPK3 alleviates mechanical allodynia and thermal hyperalgesia and decreases inflammatory cytokine protein expression in dorsal root ganglion. |
| Molecular mechanism: |
circHIPK3 interacts with miR-124 and negatively regulates miR-124 expression; miR-124 inhibition reverses circHIPK3 knockdown-mediated pain relief and inhibition of neuroinflammation. |
| Biological pathway or process: |
inflammation (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; RNA Pull-Down; Transfection; ELISA; In Vivo Animal Model |
| Clinical significance: |
circHIPK3 may act as a novel biomarker or predictive factor for neuropathic pain of type 2 diabetes; serum circHIPK3 increases with neuropathic pain severity. |
| Description: |
circHIPK3 is up-regulated in serum from patients with type 2 diabetes and neuropathic pain and in dorsal root ganglion from STZ-induced diabetic rats. The study shows that circHIPK3 promotes diabetic neuropathic pain and neuroinflammation by interacting with and negatively regulating miR-124, while intrathecal circHIPK3 shRNA alleviates pain behaviors and inflammatory cytokine expression. Clinically, higher serum circHIPK3 is associated with greater neuropathic pain severity and may serve as a biomarker or predictive factor. |
| Confidence score: |
0.6672 |