| Expression pattern: |
UP |
| Associated gene: |
HMGB1, RAGE, NF-kappaB, NF-kappaB (p65), E-Cadherin, AGO2 |
| Associated microRNA: |
miR-200a |
| Biological function: |
circRNA 101368 promotes HCC cell migration and is associated with distant metastasis, advanced TNM stage, larger tumor size, and poorer overall survival; circRNA 101368 knockdown suppresses migration and HMGB1/RAGE/NF-kappaB signaling while increasing E-Cadherin expression. |
| Molecular mechanism: |
circRNA 101368 directly binds miR-200a and negatively regulates it in an AGO2-dependent manner, thereby modulating HMGB1/RAGE signaling and downstream NF-kappaB and E-Cadherin changes to regulate HCC cell migration. |
| Biological pathway or process: |
migration (promotes); metastasis (promotes); NF-kappaB (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; Transfection; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation) |
| Clinical significance: |
circRNA 101368 overexpression is correlated with poorer prognosis, shorter overall survival, distant metastasis, advanced TNM stage, and larger tumor size in patients with HCC. |
| Description: |
circRNA 101368 is upregulated in HCC tissues and cell lines and high expression is associated with poor prognosis. Functionally, circRNA 101368 promotes HCC cell migration by binding miR-200a and modulating the HMGB1/RAGE signaling axis, with effects on HMGB1, RAGE, NF-kappaB, and E-Cadherin. |
| Confidence score: |
0.7878 |