| Expression pattern: |
DN |
| Associated gene: |
Smad7 |
| Associated microRNA: |
miR-545-3p |
| Biological function: |
inhibits proliferation, migration, invasion and epithelial-mesenchymal transition of HCC cells; suppresses tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circFGGY sponges miR-545-3p to upregulate Smad7, thereby inhibiting EMT and HCC progression |
| Biological pathway or process: |
TGF-beta/SMAD (inhibits); EMT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); H&E Staining; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circFGGY expression is associated with poor overall survival after hepatectomy; circFGGY could be a prognostic biomarker for HCC |
| Description: |
circFGGY (hsa_circ_0006633), derived from FGGY, is down-regulated in HCC and mainly localizes to the cytoplasm. It suppresses HCC proliferation, migration/invasion and EMT and inhibits xenograft tumor growth by sponging miR-545-3p to increase Smad7, thereby restraining TGF-beta/SMAD-driven EMT. Low circFGGY expression is associated with poor overall survival after hepatectomy, suggesting prognostic value. |
| Confidence score: |
0.8637 |