circRNA basic information
circBase ID: hsa_circ_0014130
Name: hsa_circ_PIP5K1A
Synonym: -
Host Gene: PIP5K1A
Genomic location(hg19): chr1:151206672-151212515:+
Genomic location(hg38): chr1:151234196-151240039:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

bladder cancer specimens; adjacent specimens; bladder cancer samples; normal samples
Cell lines:

T24; UMUC-3; 5637; EJ; SV-HUC-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: KCNJ12, GSK3beta, AKT, N-Cadherin, Twist, Vimentin, MMP-7, E-Cadherin
Associated microRNA: miR-132-3p
Biological function: hsa_circ_0014130 promotes bladder cancer cell proliferation, growth, migration, invasion, differentiation, EMT-associated marker expression, and tumor xenograft growth; knockdown inhibits malignant biological behaviors.
Molecular mechanism: hsa_circ_0014130 functions as a competitive endogenous RNA sponge for miR-132-3p, thereby upregulating KCNJ12 expression and activating EMT-associated changes and GSK3beta/AKT signaling.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); PI3K/AKT (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

High hsa_circ_0014130 expression is associated with lymph node metastasis, advanced TNM stage, aggressive characteristics, and worse survival in bladder cancer patients.
Description:

hsa_circ_0014130 is up-regulated in bladder cancer tissues and cells, and high expression is associated with lymph node metastasis, advanced TNM stage, and poor survival. Functionally, it promotes proliferation, migration, invasion, differentiation, EMT-related marker expression, and xenograft tumor growth. Mechanistically, it acts as a miR-132-3p sponge to upregulate KCNJ12 and is linked to activation of GSK3beta/AKT signaling.
Confidence score:

0.8198
Other information
Title:

CircRNA hsa_circ_0014130 function as a miR-132-3p sponge for playing oncogenic roles in bladder cancer via upregulating KCNJ12 expression.

Journal: Cell biology and toxicology
Published: 2022
PubMed ID: 34755307
Study type:

combined biological and clinical study
Data availability: GSE92675; https://www.ncbi.nlm.nih.gov/geo/
Code availability: -