| Expression pattern: |
UP |
| Associated gene: |
CDK4 |
| Associated microRNA: |
miR-198 |
| Biological function: |
Promotes melanoma cell proliferation, colony formation, invasion, glucose metabolism and tumor formation; knockdown inhibits these malignant phenotypes. |
| Molecular mechanism: |
hsa_circ_0025039 acts as a miR-198 sponge and regulates CDK4 expression through the hsa_circ_0025039/miR-198/CDK4 axis. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (other); cell cycle (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0025039 expression is associated with pathological node status, pathological metastasis status, clinical stage and poor prognosis in melanoma patients. |
| Description: |
hsa_circ_0025039 is up-regulated in melanoma tissues and cell lines, and high expression is associated with worse survival. Functionally, it promotes melanoma cell growth, invasion, glucose metabolism and xenograft tumor formation by sponging miR-198 and regulating CDK4 expression. |
| Confidence score: |
0.8709 |