| Expression pattern: |
DN |
| Associated gene: |
ATL2 |
| Associated microRNA: |
miR-214-5p |
| Biological function: |
Inhibits migration, invasion, proliferation, and metastasis of gastric cancer cells. |
| Molecular mechanism: |
hsa circ 0000993 functions as a miRNA sponge by competitively binding miR-214-5p, thereby inhibiting migration, invasion and proliferation of gastric cancer cells; it does not influence ATL2 or miR-214-5p expression levels. |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); proliferation (inhibits); metastasis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Microarray; RT-qPCR; FISH / smFISH; Transfection; Wound Healing Assay; Transwell Assay; MTT; Luciferase Reporter Assay; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
hsa_circ_0000993 is a human circRNA derived from ATL2 and is reduced in the highly aggressive gastric cancer cell line BGC-823 compared with SGC-7901. Its overexpression suppresses gastric cancer cell migration, invasion and proliferation, likely by competitively binding miR-214-5p rather than by regulating ATL2 or miR-214-5p expression levels. |
| Confidence score: |
0.7519 |