| Expression pattern: |
UP |
| Associated gene: |
WEE1, Ago2 |
| Associated microRNA: |
miR-1283 |
| Biological function: |
promotes CLL cell proliferation and survival; inhibits apoptosis; promotes cell cycle progression (G1/S) |
| Molecular mechanism: |
ceRNA sponge mechanism: circZNF91 sponges miR-1283, relieving miR-1283-mediated repression of WEE1 |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; Clinical Sample Validation; ROC Analysis; Survival Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circZNF91 has diagnostic value (AUC > 0.8) and high circZNF91 expression is associated with worse survival in CLL patients |
| Description: |
circZNF91 is up-regulated in CLL peripheral blood and CLL cell lines, where it supports proliferation and cell-cycle progression and suppresses apoptosis. Mechanistically, circZNF91 functions as a ceRNA that sponges miR-1283, thereby de-repressing WEE1 to promote malignant phenotypes. Clinically, higher circZNF91 correlates with diagnostic potential and poorer survival. |
| Confidence score: |
0.8347 |