circRNA basic information
circBase ID: -
Name: hsa_circ_CDR1
Synonym: ciRS-7 / CDR1as
Host Gene: CDR1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005595
MONDO name: laryngeal squamous cell carcinoma
Disease details: laryngeal squamous cell carcinoma / LSCC
Disease DO ID:
 2876
Disease MeSH ID:
 -
Disease NCIt ID:
 C4044
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

LSCC tissue; corresponding relative normal tissue; tumour tissues
Cell lines:

Hep2; AMC-HN-8
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CCNE1, PIK3CD
Associated microRNA: miR-7
Biological function: CDR1as functions as an oncogene in LSCC; its overexpression enhances cell vitality, proliferation, colony formation, migration, invasion, EMT phenotype, and in vivo tumour growth, while knockdown or miR-7 restoration counteracts these tumour-promoting effects.
Molecular mechanism: CDR1as acts as a miR-7 sponge and promotes LSCC progression by relieving miR-7-mediated suppression of CCNE1 and PIK3CD, thereby upregulating CCNE1 and PIK3CD mRNA and protein levels.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; IHC (Immunohistochemistry); IF (Immunofluorescence); Western Blot; In Vivo Animal Model; Survival Analysis; Cohort Study
Clinical significance:

High CDR1as levels were associated with high TNM stage, poor cellular differentiation, extensive lymph node metastasis, shorter survival time, poor clinical outcome, and potential diagnostic biomarker value in LSCC.
Description:

CDR1as is up-regulated in LSCC tissues and acts as an oncogenic circRNA. It promotes proliferation, cell-cycle progression, migration, invasion, EMT, and xenograft tumour growth by sponging miR-7 and increasing CCNE1 and PIK3CD expression. Clinically, high CDR1as expression is associated with advanced disease features and shorter survival, suggesting biomarker potential.
Confidence score:

0.6745
Other information
Title:

CDR1as is overexpressed in laryngeal squamous cell carcinoma to promote the tumour's progression via miR-7 signals.

Journal: Cell proliferation
Published: 2018
PubMed ID: 30182381
Study type:

combined biological and clinical study
Data availability: -
Code availability: -