circRNA basic information
circBase ID: hsa_circ_0003028
Name: hsa_circ_FUT8
Synonym: CircFUT8 / circ_0003028
Host Gene: FUT8
Genomic location(hg19): chr14:66028054-66028484:+
Genomic location(hg38): chr14:65561336-65561766:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0008903
MONDO name: lung cancer
Disease details: lung cancer
Disease DO ID:
 1324
Disease MeSH ID:
 -
Disease NCIt ID:
 C7377
Disease ICD11 ID:
 -
Disease OMIM ID:
 211980
Species: Human
Species details: Homo sapiens
Tissue specimen:

lung cancer tumor tissue; adjacent normal tissue; xenograft tissue
Cell lines:

A549; H1299; HEK-293T; THP-1; BEAS-2B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ENO1, JUN, CSF2, CSF1, TRAF1, CCL5, MMP9
Associated microRNA: -
Biological function: promotes proliferation, migration, and invasion; inhibits apoptosis; promotes M2 macrophage polarization; promotes tumor progression
Molecular mechanism: circFUT8 directly binds ENO1 to form an RNA-protein complex; ENO1-dependent glycolytic lactate production activates TNF signaling, driving M2 macrophage polarization
Biological pathway or process:

TNF signaling (promotes); glycolysis (promotes); immune regulation (promotes); macrophage polarization (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; RNA-seq; Bioinformatics Analysis; RNA Pull-Down; IF (Immunofluorescence); Western Blot; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); ChIP / ChIP-seq
Clinical significance:

circFUT8 was upregulated in lung cancer tissues and correlated with advanced stages and poor prognosis
Description:

circFUT8 (hsa_circ_0003028), derived from FUT8, is upregulated in lung cancer/NSCLC tissues and associates with advanced stage and poor overall survival. Functionally, it promotes tumor cell proliferation, migration and invasion while inhibiting apoptosis, and it drives an immunosuppressive microenvironment by promoting M2 macrophage polarization. Mechanistically, circFUT8 binds the RBP ENO1 in the cytoplasm, enhancing glycolysis/lactate production to activate TNF signaling and downstream genes (e.g., JUN, CSF1/CSF2, CCL5, MMP9).
Confidence score:

0.8469
Other information
Title:

Fucosyltransferase 8-Derived Circular RNA Drives M2 Polarization of Macrophages Through ENO1-TNF Signaling Axis to Promote Lung Cancer Progression.

Journal: Thoracic cancer
Published: 2025
PubMed ID: 41328768
Study type:

combined biological and clinical study
Data availability: GSE101586; GSE112214; GSE307293
Code availability: -