| Expression pattern: |
UP |
| Associated gene: |
VANGL1 mRNA, VANGL1 |
| Associated microRNA: |
miR-605-3p |
| Biological function: |
circ-VANGL1 promotes bladder cancer cell proliferation, cell-cycle progression, migration, invasion, and in vivo tumor propagation; circ-VANGL1 knockdown suppresses these malignant phenotypes. |
| Molecular mechanism: |
circ-VANGL1 acts as a competing endogenous RNA that sponges miR-605-3p, thereby relieving miR-605-3p-mediated inhibition of VANGL1 and enhancing VANGL1 expression to promote BC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; FISH / smFISH; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circ-VANGL1 could serve as a prognostic marker for patients with BC; higher circ-VANGL1 expression was associated with shorter 5-year survival, tumor stage, and metastasis. |
| Description: |
circ-VANGL1 is up-regulated in bladder cancer tissues and cell lines and high expression is associated with poorer survival and aggressive clinical features. Functionally, it promotes bladder cancer proliferation, cell-cycle progression, migration, invasion, and xenograft tumor growth. Mechanistically, circ-VANGL1 functions as a ceRNA for miR-605-3p to increase VANGL1 expression, forming the circ-VANGL1/miR-605-3p/VANGL1 axis. |
| Confidence score: |
0.8069 |