circRNA basic information
circBase ID: hsa_circ_0007534
Name: -
Synonym: circ-0007534
Host Gene: -
Genomic location(hg19): chr17:61869771-61877977:+
Genomic location(hg38): chr17:63792411-63800617:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma / OS
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS tissue specimens; corresponding normal tissues; paired adjacent normal tissue specimens; tumor tissues
Cell lines:

hFOB1.19; HOS; Saos2; MG63; U2OS
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AKT, GSK-3beta, Bcl-2, caspase-3, caspase-9, Bax, Ki67
Associated microRNA: -
Biological function: hsa_circ_0007534 promotes OS cell growth, colony formation, tumorigenesis and xenograft growth, and protects OS cells against apoptosis.
Molecular mechanism: hsa_circ_0007534 facilitates OS progression by activating the AKT/GSK-3beta signaling pathway and regulating apoptosis-related Bcl-2/caspase-3 pathway components.
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (other); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; Flow Cytometry（Non-apoptosis/cycle）; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Cohort Study; Survival Analysis
Clinical significance:

Up-regulated hsa_circ_0007534 is associated with larger tumor size and poorer differentiation degree, and high expression predicts shorter overall survival and unfavorable prognosis in OS patients.
Description:

hsa_circ_0007534 is up-regulated in osteosarcoma tissues and cell lines, and its high expression is associated with larger tumor size, poorer differentiation and worse overall survival. Functionally, it promotes OS cell proliferation, colony formation and xenograft growth while inhibiting apoptosis, at least partly through AKT/GSK-3beta signaling and Bcl-2/caspase-3-related apoptotic regulation.
Confidence score:

0.6742
Other information
Title:

Overexpression of hsa_circ_0007534 predicts unfavorable prognosis for osteosarcoma and regulates cell growth and apoptosis by affecting AKT/GSK-3beta signaling pathway.

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Published: 2018
PubMed ID: 30142548
Study type:

combined biological and clinical study
Data availability: -
Code availability: -