| Expression pattern: |
UP |
| Associated gene: |
EGFR, LDHA, HK2, GLUT1, PKM2, ERK1/2, PCSK6 |
| Associated microRNA: |
miR-876-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, glycolysis, tumor growth, and cisplatin resistance; high expression is associated with poor prognosis. |
| Molecular mechanism: |
hsa_circ_103089 functions as a ceRNA/miRNA sponge for miR-876-5p, thereby increasing EGFR expression, activating EGFR/ERK signaling and PKM2 phosphorylation/nuclear translocation, and promoting glycolytic gene/protein expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes); ERK (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; TUNEL; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_103089 expression correlated with poor prognosis in NSCLC patients and may serve as a potential biomarker for predicting malignant progression and chemotherapeutic response. |
| Description: |
hsa_circ_103089 is upregulated in NSCLC tissues and cell lines and is associated with poorer overall survival. Functionally, it promotes malignant phenotypes, glycolysis, tumor growth, and cisplatin resistance. Mechanistically, it sponges miR-876-5p to enhance EGFR expression and downstream ERK/PKM2-mediated glycolytic regulation. |
| Confidence score: |
0.8659 |