| Expression pattern: |
UP |
| Associated gene: |
ErbB3 / erb-b2 receptor tyrosine kinase 3 |
| Associated microRNA: |
miR-22 |
| Biological function: |
Promotes LAC cell proliferation and tumour growth; knockdown suppresses proliferation of A549 and PC9 cells and inhibits tumour growth in vivo. |
| Molecular mechanism: |
hsa_circ_0012673 functions as a cytoplasmic ceRNA sponge for miR-22, relieving miR-22-mediated repression of ErbB3 and increasing ErbB3 protein levels. |
| Biological pathway or process: |
proliferation (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_0012673 expression was significantly associated with tumour size in LAC patients. |
| Description: |
hsa_circ_0012673 is up-regulated in lung adenocarcinoma tissues and cell lines, and its expression is associated with tumour size. It primarily localizes in the cytoplasm and promotes LAC proliferation through a ceRNA mechanism by sponging miR-22 and increasing ErbB3 protein expression; knockdown suppresses proliferation in vitro and tumour growth in a xenograft model. |
| Confidence score: |
0.8314 |