circRNA basic information
circBase ID: -
Name: hsa_circ_TUBGCP4
Synonym: circTUBGCP4 / circ101501
Host Gene: TUBGCP4
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum exosomes; CRC tissue; paired adjacent normal tissue
Cell lines:

HCT116; SW480; 293 T; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PDK2, AGO2, Akt
Associated microRNA: miR-146b-3p
Biological function: promotes vascular endothelial cell migration, tube formation, tip cell formation and colorectal cancer metastasis; promotes angiogenesis
Molecular mechanism: circTUBGCP4 acts as a miRNA sponge for miR-146b-3p, upregulates PDK2 and activates Akt signaling pathway
Biological pathway or process:

Akt signaling (promotes); PI3K/AKT (promotes); angiogenesis (promotes); migration (promotes); proliferation (other); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
H
M
Validation methods:

Microarray; RNase R Treatment; RT-qPCR; ISH (In Situ Hybridization); Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; Western Blot; IF (Immunofluorescence); Transwell Assay; Wound Healing Assay; Tube Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

High circTUBGCP4 expression is associated with metastasis and angiogenesis markers (CD31/CD34) in CRC
Description:

In CRC, exosomal circTUBGCP4 is up-regulated in metastatic settings and is transferred to endothelial cells, where it promotes migration, tube formation, and tip cell formation, thereby enhancing angiogenesis and metastasis. Mechanistically, it functions as a ceRNA to sponge miR-146b-3p, upregulate PDK2, and activate Akt signaling.
Confidence score:

0.8794
Other information
Title:

Exosomal circTUBGCP4 promotes vascular endothelial cell tipping and colorectal cancer metastasis by activating Akt signaling pathway.

Journal: Journal of experimental & clinical cancer research : CR
Published: 2023
PubMed ID: 36793126
Study type:

combined biological and clinical study
Data availability: GSE126094; GSE147597; GSE71187; GSE87211; GSE25071; GSE39582
Code availability: -