circRNA basic information
circBase ID: hsa_circ_0007312
Name: -
Synonym: circ7312 / circ-0007312
Host Gene: -
Genomic location(hg19): chr22:24698162-24709434:+
Genomic location(hg38): chr22:24302194-24313466:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005061
MONDO name: lung adenocarcinoma
Disease details: lung adenocarcinoma / LUAD
Disease DO ID:
 3910
Disease MeSH ID:
 C538231
Disease NCIt ID:
 C3512
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal tissues
Cell lines:

PC9; PC9/ER
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MAPK1
Associated microRNA: miR-764
Biological function: promotes osimertinib resistance; inhibits pyroptosis and apoptosis; promotes migration and invasion; promotes tumor growth in vivo
Molecular mechanism: circ7312 acts as a ceRNA to sponge miR-764, thereby regulating MAPK1 expression and modulating pyroptosis and apoptosis associated with osimertinib resistance.
Biological pathway or process:

pyroptosis (inhibits); apoptosis (inhibits); migration (promotes); invasion (promotes); proliferation (promotes); chemoresistance (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

circ7312 is upregulated in LUAD tumor tissues and high circ7312 expression is related to poor prognosis (longer DFS/OS in low-expression group).
Description:

hsa_circ_0007312 (circ7312) is upregulated in LUAD tumor tissues and is associated with poor prognosis. Functionally, circ7312 promotes osimertinib (third-generation EGFR-TKI) resistance and tumor aggressiveness by sponging miR-764 to upregulate MAPK1, thereby reducing pyroptosis and apoptosis; circ7312 knockdown restores drug sensitivity in vitro and in a xenograft model.
Confidence score:

0.7126
Other information
Title:

Hsa_circ_0007312 Promotes Third-Generation Epidermal Growth Factor Receptor-Tyrosine Kinase Inhibitor Resistance through Pyroptosis and Apoptosis via the MiR-764/MAPK1 Axis in Lung Adenocarcinoma Cells.

Journal: Journal of Cancer
Published: 2022
PubMed ID: 35812182
Study type:

combined biological and clinical study
Data availability: Genomics of Drug Sensitivity in Cancer Project (https://www.cancerrxgene.org/); Cancer Cell Line Encyclopedia (https://sites.broadinstitute.org/ccle/)
Code availability: -