circRNA basic information
circBase ID: hsa_circ_0007509
Name: hsa_circ_PPP4R1
Synonym: circPPP4R1
Host Gene: PPP4R1
Genomic location(hg19): chr18:9583114-9595151:-
Genomic location(hg38): chr18:9583116-9595153:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

H1975; H1975-OR
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: -
Biological function: Promotes proliferation, migration and angiogenesis and inhibits apoptosis in osimertinib-resistant NSCLC cells (H1975-OR); its knockdown increases sensitivity to osimertinib.
Molecular mechanism: Not specified (functional effects shown by siRNA knockdown; no miRNA/protein axis reported).
Biological pathway or process:

proliferation (promotes); migration (promotes); angiogenesis (promotes); apoptosis (inhibits); drug resistance (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Tube Formation Assay; Bioinformatics Analysis
Clinical significance:

-
Description:

circPPP4R1 (hsa_circ_0007509) is up-regulated in osimertinib-resistant H1975-OR cells. Functionally, circPPP4R1 supports the resistant phenotype by promoting proliferation, migration and angiogenesis and suppressing apoptosis; silencing it increases sensitivity to osimertinib in H1975-OR cells.
Confidence score:

0.6873
Other information
Title:

Characterization of circRNAs in established osimertinib‑resistant non‑small cell lung cancer cell lines.

Journal: International journal of molecular medicine
Published: 2023
PubMed ID: 37681495
Study type:

biological research
Data availability: PRJNA1000087; PRJNA1000497
Code availability: -