circRNA basic information
circBase ID: hsa_circ_0124644
Name: hsa_circ_ROBO2
Synonym: circ_ROBO2
Host Gene: ROBO2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005010
MONDO name: coronary artery disorder
Disease details: coronary artery disease / CAD
Disease DO ID:
 3393
Disease MeSH ID:
 D003324
Disease NCIt ID:
 C26732
Disease ICD11 ID:
 1059873720
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

blood samples; plasma
Cell lines:

HASMCs
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: TRAF6, NF-kappaB signaling
Associated microRNA: miR-149
Biological function: Promotes proliferation and migration, suppresses apoptosis, and activates NF-kappaB signaling in PDGF-BB-treated HASMCs in the context of CAD.
Molecular mechanism: circ_ROBO2 acts as a sponge of miR-149 and positively regulates TRAF6/NF-kappaB signaling.
Biological pathway or process:

NF-kappaB (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; Bioinformatics Analysis
Clinical significance:

Highly expressed in peripheral blood/plasma of CAD patients and may provide a basis for application in CAD diagnosis.
Description:

This study reports that circ_ROBO2/Hsa_circ_0124644 is upregulated in plasma from CAD patients and in PDGF-BB-treated HASMCs. Functionally, circ_ROBO2 promotes HASMC proliferation and migration while suppressing apoptosis. Mechanistically, circ_ROBO2 sponges miR-149 and activates the TRAF6/NF-kappaB signaling pathway, suggesting a role in CAD progression and potential diagnostic relevance.
Confidence score:

0.7032
Other information
Title:

Circ_ROBO2/miR-149 Axis Promotes the Proliferation and Migration of Human Aortic Smooth Muscle Cells by Activating NF-kappaB Signaling.

Journal: Cytogenetic and genome research
Published: 2021
PubMed ID: 34649241
Study type:

combined biological and clinical study
Data availability: All data generated or analysed during this study are included in this article; datasets available from the corresponding author on reasonable request
Code availability: -