circRNA basic information
circBase ID: -
Name: hsa_circ_NEK6
Synonym: CircRNA_104916
Host Gene: NEK6
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

human CRC samples; paired adjacent noncancerous specimen; tumorous tissues; adjacent normal tissues

Cell lines:

Caco-2; LoVo; SW480; HT-29; HCT116; FHC

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
DN
Associated gene: cyclin D1, cyclin B1, E-cadherin, N-cadherin, Caspase-3, Cleaved Caspase-3, Bax, Bcl-2
Associated microRNA: -
Biological function: CircRNA_104916 functions as a tumor suppressor in colorectal cancer by inhibiting cell proliferation, migration, invasion and EMT, while promoting apoptosis and G2/M phase arrest.
Molecular mechanism: CircRNA_104916 overexpression activates apoptosis-related proteins and cell-cycle checkpoint protein changes, and inhibits EMT marker changes, resulting in suppressed proliferation, migration and invasion.
Biological pathway or process:

apoptosis (promotes); cell cycle (other); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); other pathway/process (other)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis

Clinical significance:

CircRNA_104916 is a potential biomarker and therapeutic target for colorectal cancer; higher expression is associated with better disease-free survival and circRNA_104916 independently predicts prognosis.

Description:

CircRNA_104916 is downregulated in colorectal cancer tissues and cell lines, and higher expression is associated with better disease-free survival. Functional experiments show that circRNA_104916 overexpression suppresses CRC proliferation, migration, invasion and EMT, while promoting apoptosis and G2/M phase arrest. The study supports circRNA_104916 as a potential tumor suppressor, biomarker and therapeutic target in CRC.

Confidence score:

0.6589

Other information
Title:

CircRNA_104916 regulates migration, apoptosis and epithelial-mesenchymal transition in colon cancer cells.

Journal: Frontiers in bioscience (Landmark edition)
Published: 2019
PubMed ID: 30844715
Study type:

combined biological and clinical study

Data availability: -
Code availability: -