circRNA basic information
circBase ID: hsa_circ_0005615
Name: hsa_circ_NFATC3
Synonym: circ_0005615
Host Gene: -
Genomic location(hg19): chr16:68155889-68157024:+
Genomic location(hg38): chr16:68121986-68123121:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; matched adjacent normal tissues; xenograft tissues

Cell lines:

LOVO; SW480; NCM460

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: NOTCH1
Associated microRNA: miR-665
Biological function: promotes radioresistance, proliferation, migration and invasion; inhibits apoptosis; circ_0005615 knockdown enhances radiosensitivity in vitro and in vivo
Molecular mechanism: circ_0005615 acts as a miRNA sponge (ceRNA) for miR-665, thereby increasing NOTCH1 expression and promoting radioresistance
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); radioresistance (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; Colony Formation Assay; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis

Clinical significance:

circ_0005615 level was higher in the high-radiosensitive (recurrent after radiation treatment) group than in the less-radiosensitive group; suggested as a potential biomarker for CRC radiotherapy

Description:

circ_0005615 is up-regulated in colorectal cancer and mainly localized in the cytoplasm. It promotes radioresistance by sponging miR-665 to upregulate NOTCH1, thereby supporting CRC cell survival/proliferation and migration/invasion while reducing apoptosis; its knockdown increases radiosensitivity in vitro and in a LOVO xenograft model.

Confidence score:

0.6837

Other information
Title:

Knockdown of circ_0005615 enhances the radiosensitivity of colorectal cancer by regulating the miR-665/NOTCH1 axis.

Journal: Open medicine (Warsaw, Poland)
Published: 2023
PubMed ID: 37727322
Study type:

combined biological and clinical study

Data availability: The analyzed datasets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -