| Expression pattern: |
UP |
| Associated gene: |
USP7, NRF2, HIF-1alpha |
| Associated microRNA: |
- |
| Biological function: |
Promotes breast cancer progression, invasion and metastasis by inducing M2-like macrophage polarisation. |
| Molecular mechanism: |
Exosomal circ-0100519 is delivered to macrophages and acts as a scaffold to enhance USP7-NRF2 interaction, promoting USP7-mediated deubiquitination and stabilization of NRF2; HIF-1alpha enhances circ-0100519 transcription. |
| Biological pathway or process: |
metastasis (promotes); invasion (promotes); proliferation (promotes); macrophage polarization (promotes); ubiquitination (inhibits); other pathway/process (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; ISH (In Situ Hybridization); Survival Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Co-IP; Luciferase Reporter Assay; ChIP / ChIP-seq; TUNEL; IF (Immunofluorescence); H&E Staining |
| Clinical significance: |
Upregulated in BC tumour tissues and serum exosomes; related to poor prognosis; potential biomarker for BC diagnosis and prognosis. |
| Description: |
circ-0100519 is up-regulated in breast cancer tissues and enriched in serum exosomes, where higher levels associate with poor prognosis. Tumor-secreted exosomal circ-0100519 is taken up by macrophages and promotes M2 polarization by scaffolding USP7 and NRF2 to enhance NRF2 deubiquitination/stability, thereby promoting breast cancer invasion and metastasis; HIF-1alpha upregulates circ-0100519 transcription. |
| Confidence score: |
0.8886 |