| Expression pattern: |
UP |
| Associated gene: |
HMGB3 |
| Associated microRNA: |
miR-545-3p, miR-506-3p |
| Biological function: |
circFOXO3 promotes NSCLC cell proliferation, migration and invasion, and promotes tumor growth in vivo. |
| Molecular mechanism: |
circFOXO3 acts as a ceRNA by sponging miR-545-3p and miR-506-3p, preventing them from targeting the 3′-UTR of HMGB3 mRNA and resulting in HMGB3 upregulation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Western Blot; Luciferase Reporter Assay; CCK8; Transfection; Transwell Assay; In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circFOXO3 expression is associated with lower survival rates, lymph node metastasis and tumor stage, and circFOXO3 is proposed as a potential therapeutic target for NSCLC. |
| Description: |
circFOXO3 is up-regulated in NSCLC tissues and cell lines, and high expression is associated with poorer survival and aggressive clinicopathological features. Functionally, circFOXO3 promotes proliferation, migration, invasion and xenograft tumor growth by sponging miR-545-3p and miR-506-3p, thereby relieving repression of HMGB3. |
| Confidence score: |
0.7113 |