circRNA basic information
circBase ID: hsa_circ_0077232
Name: hsa_circ_NT5E
Synonym: circNT5E
Host Gene: NT5E
Genomic location(hg19): chr6:86180954-86205509:+
Genomic location(hg38): chr6:85471236-85495791:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0018177
MONDO name: glioblastoma
Disease details: glioblastoma / GBM
Disease DO ID:
 3068
Disease MeSH ID:
 D005909
Disease NCIt ID:
 C3058
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissue samples; adjacent normal tissues; glioblastoma tissues; normal brain tissues; GBM tumor tissues
Cell lines:

U87; U251
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ADARB2, ADAR1, NT5E pre-mRNA, NT5E, SOX4, PI3KCA, p-Akt, p-Smad2, CDK4, AURKA, MMP-9, EGFR
Associated microRNA: miR-422a, miR-124
Biological function: circNT5E promotes glioblastoma cell growth, proliferation, invasion, migration, and tumor formation, and inhibits apoptosis.
Molecular mechanism: circNT5E is formed from the NT5E genome and regulated by ADARB2; it functions as a ceRNA/miRNA sponge, particularly for miR-422a, thereby affecting NT5E, SOX4, PI3KCA, p-Akt and p-Smad2 signaling, and also regulates CDK4 and AURKA through miR-124.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); PI3K/AKT (promotes); TGF-beta/SMAD (promotes); other pathway/process (promotes)
Detected method:
Q
H
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Microarray; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

circNT5E expression significantly increased as the clinical classification increased, suggesting association with glioma grade and potential clinical relevance as a biomarker or therapeutic target.
Description:

circNT5E is an NT5E-derived human circular RNA that is up-regulated in glioblastoma and increases with glioma clinical grade. It promotes glioblastoma growth, proliferation, invasion, migration, and xenograft tumor formation while inhibiting apoptosis, mainly by acting as a sponge for tumor-suppressive miR-422a and regulating NT5E, SOX4, PI3KCA and downstream p-Akt/p-Smad2 signaling. Its biogenesis is promoted by ADARB2 binding to NT5E pre-mRNA flanking intronic elements.
Confidence score:

0.8794
Other information
Title:

CircNT5E Acts as a Sponge of miR-422a to Promote Glioblastoma Tumorigenesis.

Journal: Cancer research
Published: 2018
PubMed ID: 29967262
Study type:

combined biological and clinical study
Data availability: GSE109569
Code availability: -