| Expression pattern: |
DN |
| Associated gene: |
PTEN, AKT, p-AKT |
| Associated microRNA: |
miR-3171 |
| Biological function: |
CircHIAT1 suppresses HCC cell growth, inhibits cell proliferation, and promotes cell apoptosis in vitro and in vivo. |
| Molecular mechanism: |
CircHIAT1 acts as a miR-3171 sponge to positively regulate PTEN, thereby suppressing HCC cell growth through the circHIAT1/miR-3171/PTEN axis. |
| Biological pathway or process: |
proliferation (inhibits); apoptosis (promotes); ceRNA regulation (promotes); PI3K/AKT (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; circRIP; Luciferase Reporter Assay; Microarray; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Downregulation of circHIAT1 was positively correlated with poor overall survival of HCC patients, suggesting prognostic significance. |
| Description: |
CircHIAT1 is down-regulated in HCC tissues and cell lines, and lower circHIAT1 expression is associated with poorer overall survival. Functionally, circHIAT1 suppresses HCC cell proliferation and tumor growth while promoting apoptosis. Mechanistically, cytoplasmic circHIAT1 acts as a sponge for miR-3171 to upregulate PTEN and modulate downstream AKT phosphorylation. |
| Confidence score: |
0.7896 |