circRNA basic information
circBase ID: hsa_circ_0000775
Name: hsa_circ_KIF18B
Synonym: circ_0000775
Host Gene: KIF18B
Genomic location(hg19): chr17:43012303-43012398:-
Genomic location(hg38): chr17:44934935-44935030:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; paracancerous tissues; tumor tissues
Cell lines:

THLE-2; HuH-7; Hep 3B; Li-7; 293 T
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: IGF2BP2, CDC27, TCF7L2, KIF18B promoter
Associated microRNA: -
Biological function: promotes HC cell migration, invasion and EMT
Molecular mechanism: TCF7L2 transcriptionally activates circ_0000775; cytoplasmic circ_0000775 recruits IGF2BP2 to maintain CDC27 mRNA stability via m6A modification
Biological pathway or process:

migration (promotes); invasion (promotes); EMT (promotes); mRNA stability (promotes); m6A modification (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

circ_0000775 is an up-regulated KIF18B-derived circRNA in hepatic carcinoma models. TCF7L2 activates its transcription, and cytoplasmic circ_0000775 recruits the RBP IGF2BP2 to stabilize CDC27 mRNA via m6A-related regulation, thereby promoting HC cell migration, invasion and EMT.
Confidence score:

0.813
Other information
Title:

Circ_0000775 promotes the migration, invasion and EMT of hepatic carcinoma cells by recruiting IGF2BP2 to stabilize CDC27.

Journal: Pathology, research and practice
Published: 2022
PubMed ID: 35561648
Study type:

combined biological and bioinformatics study
Data availability: GSE94508; Supplementary data associated with this article can be found in the online version at doi:10.1016/j.prp.2022.153908
Code availability: -