| Expression pattern: |
UP |
| Associated gene: |
HIF1A, Ago2 |
| Associated microRNA: |
miR-29b |
| Biological function: |
promotes NSCLC cell migration, invasion, proliferation, and angiogenesis/tube formation under hypoxia; counteracts PESV-induced repression of malignancy; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circ_0016760 sponges miR-29b to relieve suppression of HIF1A, increasing HIF1A expression under hypoxia |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Transwell Assay; Colony Formation Assay; MTT; Tube Formation Assay; Annexin V/PI Flow Cytometry; IHC (Immunohistochemistry); In Vivo Animal Model |
| Clinical significance: |
- |
| Description: |
circ_0016760 (from host gene SNAP47) is up-regulated in NSCLC tissues/cells and promotes hypoxia-associated malignant phenotypes (migration, invasion, proliferation) and angiogenesis/tube formation. Mechanistically, it sponges miR-29b, thereby increasing HIF1A expression; PESV suppresses NSCLC malignancy under hypoxia partly by downregulating circ_0016760, and circ_0016760 overexpression rescues these inhibitory effects in vitro and in xenografts. |
| Confidence score: |
0.7371 |