circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: Circular RNA HIPK3 / circHIPK3
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005411
MONDO name: gallbladder cancer
Disease details: gallbladder cancer
Disease DO ID:
 3121
Disease MeSH ID:
 -
Disease NCIt ID:
 C7481
Disease ICD11 ID:
 922082116
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human gallbladder cancer tissues; surrounding normal gallbladder epithelial tissues; gallbladder cancer tissues; non-cancerous surrounding gallbladder epithelial tissues
Cell lines:

Mz-ChA-1; QBC939; GBC-SD; transformed immortal human gallbladder epithelial cells
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK1, CDK6
Associated microRNA: miR-124
Biological function: Promotes gallbladder cancer cell survival, proliferation and colony formation, and inhibits apoptosis; circHIPK3 silencing inhibits cancer cell viability and proliferation while inducing apoptosis.
Molecular mechanism: circHIPK3 sponges and inhibits miR-124, leading to increased expression of miR-124 targets ROCK1 and CDK6.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; BrdU; Colony Formation Assay; TUNEL; Western Blot
Clinical significance:

The overexpressed circHIPK3 could be a novel therapeutic target and diagnosis marker of human gallbladder cancer.
Description:

circHIPK3 is upregulated in human gallbladder cancer cells and tissues. Functionally, it promotes gallbladder cancer cell survival, proliferation and colony formation while suppressing apoptosis. Mechanistically, circHIPK3 acts as a miR-124 sponge, increasing ROCK1 and CDK6 expression, and may serve as a therapeutic target and diagnostic marker.
Confidence score:

0.5434
Other information
Title:

Circular RNA HIPK3 promotes gallbladder cancer cell growth by sponging microRNA-124.

Journal: Biochemical and biophysical research communications
Published: 2018
PubMed ID: 29928876
Study type:

combined biological and clinical study
Data availability: Transparency document related to this article can be found online at https://doi.org/10.1016/j.bbrc.2018.06.088.
Code availability: -