| Expression pattern: |
DN |
| Associated gene: |
METTL14, AGO2, JMY, p53, CD8A |
| Associated microRNA: |
miR-7108-3p |
| Biological function: |
Suppresses NSCLC cell proliferation and migration; enhances CD3+CD8+ T cell-mediated cytotoxicity/anti-tumor immunity. |
| Molecular mechanism: |
m6A modification by METTL14 decreases circZNF548; circZNF548 sponges miR-7108-3p (AGO2-associated) and reduces exosomal miR-7108-3p, de-repressing JMY and activating the JMY-p53 apoptotic pathway; promotes CD8+ T cell activation via lowering exosomal miR-7108-3p. |
| Biological pathway or process: |
p53 signaling (promotes); apoptosis (promotes); proliferation (inhibits); migration (inhibits); ceRNA regulation (promotes); immune regulation (promotes) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; ISH (In Situ Hybridization); RNase R Treatment; Actinomycin D / DRB Stability Assay; MeRIP / MeRIP-seq; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; FISH / smFISH; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle）; Annexin V/PI Flow Cytometry; ELISA; In Vivo Animal Model; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Higher circZNF548 levels correlate with improved patient survival in NSCLC. |
| Description: |
circZNF548 is down-regulated in NSCLC and acts as a tumor suppressor. It sponges miR-7108-3p and lowers exosomal miR-7108-3p, thereby increasing JMY and activating p53-dependent apoptosis while enhancing CD8+ T-cell cytotoxicity, which together inhibit NSCLC growth and migration. |
| Confidence score: |
0.8793 |