| Expression pattern: |
UP |
| Associated gene: |
WNT2B, E2F1 |
| Associated microRNA: |
miR-185-3p |
| Biological function: |
Promotes proliferation, migration, and invasion; inhibits apoptosis; promotes cell cycle progression (reduces G1 arrest); promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-185-3p, affecting miR-185-3p targets (E2F1 and WNT2B). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; MTT; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model |
| Clinical significance: |
circ_0088233 level correlated with TNM stage; implicated as a potential therapeutic target for prostate cancer. |
| Description: |
circ_0088233 is up-regulated in prostate cancer tissues and cell lines and correlates with TNM stage. It promotes prostate cancer cell proliferation, migration, invasion, cell cycle progression and tumor growth while inhibiting apoptosis, mainly by sponging miR-185-3p and affecting downstream targets including E2F1 and WNT2B. |
| Confidence score: |
0.8122 |