| Expression pattern: |
UP |
| Associated gene: |
TIPARP, HECTD1, GFAP, MAP1LC3B, BECN1, SQSTM1 |
| Associated microRNA: |
MIR142 |
| Biological function: |
Promotes cerebral infarction, neurological deficits, astrocyte activation and astrocyte autophagy in ischemic stroke; knockdown decreases infarct areas, attenuates neuronal deficits, and ameliorates astrocyte activation. |
| Molecular mechanism: |
circHECTD1 acts as an endogenous MIR142 sponge to inhibit MIR142 activity and regulate TIPARP expression, thereby promoting astrocyte activation through autophagy. |
| Biological pathway or process: |
ceRNA regulation (promotes); autophagy (promotes); apoptosis (other); proliferation (other); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circHECTD1 can serve as a novel biomarker of and therapeutic target for stroke; increased plasma circHECTD1 was detected in AIS patients. |
| Description: |
circHECTD1 is up-regulated in ischemic brain tissues from tMCAO mice and in plasma from AIS patients. It promotes astrocyte activation and autophagy through a circHECTD1-MIR142-TIPARP mechanism, and its knockdown reduces infarct size and improves neurological outcomes, supporting its potential as a biomarker and therapeutic target for ischemic stroke. |
| Confidence score: |
0.8173 |