| Expression pattern: |
UP |
| Associated gene: |
beta-TrCP, c-Myc, SRSF1 |
| Associated microRNA: |
- |
| Biological function: |
promotes migration, invasion and metastasis of NPC cells |
| Molecular mechanism: |
circPVT1 binds beta-TrCP, competitively blocks beta-TrCP-c-Myc interaction, inhibits beta-TrCP-mediated ubiquitination/proteasomal degradation of c-Myc, stabilizes c-Myc; c-Myc upregulates SRSF1 and recruits it to enhance circPVT1 biosynthesis, forming a positive feedback loop |
| Biological pathway or process: |
metastasis (promotes); migration (promotes); invasion (promotes); EMT (promotes); ubiquitination (inhibits); cytoskeleton remodeling (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; ISH (In Situ Hybridization); Clinical Sample Validation; Wound Healing Assay; Transwell Assay; CCK8; Colony Formation Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Co-IP; IF (Immunofluorescence); Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circPVT1 expression was positively correlated with poor prognosis, clinical stages, N stages, and distant metastasis in NPC patients; may be a potential biomarker for the detection of NPC metastasis |
| Description: |
circPVT1 is up-regulated in nasopharyngeal carcinoma and promotes migration, invasion, and metastasis. It binds beta-TrCP to inhibit beta-TrCP-mediated ubiquitination and degradation of c-Myc, stabilizing c-Myc to drive cytoskeleton remodeling/cell adhesion changes; c-Myc and SRSF1 further enhance circPVT1 biogenesis, forming a beta-TrCP/c-Myc/SRSF1 positive feedback loop. |
| Confidence score: |
0.8863 |