| Expression pattern: |
UP |
| Associated gene: |
FAF1, TP73, AGO2 |
| Associated microRNA: |
miR-345-3p |
| Biological function: |
Promotes TNF-alpha-induced NPCs PAoptosis (apoptosis/pyroptosis), inflammatory response, growth inhibition, and ECM degradation; more strongly promotes apoptosis than circ_0004354. |
| Molecular mechanism: |
ceRNA mechanism: competitively adsorbs miR-345-3p in RISC (AGO2-associated), thereby positively regulating miR-345-3p target genes FAF1/TP73 and activating CASP3-GSDME-mediated PAoptosis. |
| Biological pathway or process: |
apoptosis (promotes); pyroptosis (promotes); inflammation (promotes); proliferation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Clinical Sample Validation |
| Clinical significance: |
Expression was correlated with the severity of IVDD. |
| Description: |
circ_0040039 is up-regulated in IVDD NP tissues and promotes TNF-alpha-induced NPC PAoptosis, inflammatory response, growth inhibition, and ECM degradation. Mechanistically, it acts as a ceRNA by binding miR-345-3p (AGO2/RISC-associated) to increase FAF1 and TP73, with a stronger pro-apoptotic effect relative to circ_0004354. |
| Confidence score: |
0.8344 |