| Expression pattern: |
UP |
| Associated gene: |
Ago2, HBEGF, TGF-beta, Smad2/3, P-Smad2/3 |
| Associated microRNA: |
miR-4659a-3p, miR-4659b-3p |
| Biological function: |
Promotes GC cell proliferation, migration, invasion and metastasis; exosomal delivery enhances proliferation and migration of co-cultured GC cells. |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-4659a/b-3p, thereby increasing HBEGF and activating TGF-beta/Smad expression/signaling to promote GC progression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); TGF-beta/SMAD (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Western Blot; RIP (RNA Immunoprecipitation); FISH / smFISH; Luciferase Reporter Assay; Transfection; ROC Analysis; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum exosomal hsa_circ_000200 shows diagnostic ability for GC and is associated with invasion depth, TNM staging, and distal metastasis; high tissue expression is related to shorter survival time. |
| Description: |
hsa_circ_000200 is up-regulated in gastric cancer tissues, serum, and serum exosomes, and high levels associate with metastasis-related clinicopathologic features and shorter survival. Functionally, it promotes GC cell proliferation, migration and invasion, and can be transferred via exosomes to enhance malignant behaviors of recipient cells. Mechanistically, it sponges miR-4659a/b-3p to upregulate HBEGF and promote TGF-beta/Smad signaling. |
| Confidence score: |
0.8655 |