| Expression pattern: |
UP |
| Associated gene: |
FOXM1 |
| Associated microRNA: |
miR-149 |
| Biological function: |
circHIPK3 promotes NSCLC cell proliferation, migration, invasion, tumorigenesis and metastasis, and inhibits apoptosis; circHIPK3 silencing suppresses proliferation, migration and in vivo tumor growth. |
| Molecular mechanism: |
circHIPK3 functions as a miR-149 sponge and regulates NSCLC cell functions through miR-149-mediated FOXM1 expression regulation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model |
| Clinical significance: |
circHIPK3 upregulation is a potential biomarker for NSCLC and circHIPK3 silencing is of therapeutic value in vivo. |
| Description: |
This study reports that circHIPK3 is up-regulated in NSCLC tissues and cell lines. Functionally, circHIPK3 promotes NSCLC cell proliferation, migration, invasion, tumor growth and metastasis, while its knockdown suppresses these malignant phenotypes. Mechanistically, circHIPK3 acts through a miR-149/FOXM1 regulatory axis and is proposed as a potential biomarker and therapeutic target for NSCLC. |
| Confidence score: |
0.8168 |