| Expression pattern: |
UP |
| Associated gene: |
FOXF1 |
| Associated microRNA: |
miR-1253 |
| Biological function: |
circNASP promotes malignant behaviors of osteosarcoma cells, including proliferation, cell cycle progression and invasion; circNASP expression is positively correlated with tumor size and metastasis in OS patients. |
| Molecular mechanism: |
circNASP acts as a sponge of miR-1253, inhibits miR-1253 availability, and promotes FOXF1 expression through the miR-1253/FOXF1 pathway. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot |
| Clinical significance: |
circNASP expression was positively correlated with tumor size and metastasis in OS patients; circNASP/miR-1253/FOXF1 axis might be a potential therapeutic target. |
| Description: |
circNASP (hsa_circ_0092340), derived from NASP, is up-regulated in osteosarcoma tissues and cell lines. It promotes osteosarcoma cell proliferation, cell cycle progression and invasion by sponging miR-1253 and thereby increasing FOXF1 expression. Clinically, higher circNASP expression correlates with larger tumor size and metastasis, suggesting therapeutic relevance. |
| Confidence score: |
0.6815 |