circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: circHIPK3 / circRNA Homeodomain-Interacting Protein Kinase 3
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005411
MONDO name: gallbladder cancer
Disease details: Gallbladder cancer / GBC
Disease DO ID:
 3121
Disease MeSH ID:
 -
Disease NCIt ID:
 C7481
Disease ICD11 ID:
 922082116
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GBC tissues; paracancerous tissues / paracarcinoma tissues
Cell lines:

NOZ; GBC-SD; HIBEpiCs; HUVECs
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ALYREF, FASN, SKP2
Associated microRNA: miR-124
Biological function: promotes proliferation, migration, invasion and angiogenesis; inhibits apoptosis; promotes tumor growth in vivo
Molecular mechanism: circHIPK3 binds ALYREF, inhibits ubiquitination-mediated degradation of ALYREF, thereby stabilizing FASN mRNA
Biological pathway or process:

angiogenesis (promotes); apoptosis (inhibits); proliferation (promotes); migration (promotes); invasion (promotes); mRNA stability (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; Co-IP; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis; Clinical Sample Validation
Clinical significance:

Elevated circHIPK3 expression correlates with TNM stages and lymph node metastasis; proposed as a prognostic biomarker and therapeutic target
Description:

circHIPK3 is up-regulated in gallbladder cancer and promotes malignant phenotypes (proliferation, migration/invasion, angiogenesis) while inhibiting apoptosis. Mechanistically, it binds and stabilizes the RBP ALYREF by reducing its ubiquitination, which in turn stabilizes FASN mRNA and increases FASN expression, driving angiogenesis and anti-apoptotic effects.
Confidence score:

0.8635
Other information
Title:

CircHIPK3's dual role in promoting angiogenesis and inhibiting apoptosis through FASN mRNA stabilization in gallbladder cancer.

Journal: Clinics (Sao Paulo, Brazil)
Published: 2025
PubMed ID: 40466438
Study type:

combined biological and clinical study
Data availability: GSE148561; MiOncoCirc; ENCORI; GEPIA2; The datasets used and/or analyzed during the present study are available from the corresponding author upon reasonable request.
Code availability: -