| Expression pattern: |
UP |
| Associated gene: |
YTHDF3, eIF4G2, eIF4A, eIF4B, LATS1, YAP/TEAD1 complex |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC cell migration and invasion; facilitates liver metastasis |
| Molecular mechanism: |
circ-YAP is translated (m6A-dependent) into YAP-220aa; YTHDF3 binds m6A-modified circ-YAP and recruits eIF4G2 translation initiation complex; YAP-220aa competitively binds LATS1, reduces YAP phosphorylation, promotes YAP nuclear translocation and activation of pro-metastasis genes; YAP/TEAD complex transactivates circ-YAP forming a positive feedback loop |
| Biological pathway or process: |
Hippo/YAP (promotes); migration (promotes); invasion (promotes); metastasis (promotes); m6A modification (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Microarray; RNA-seq; FISH / smFISH; ISH (In Situ Hybridization); RNase R Treatment; Actinomycin D / DRB Stability Assay; Sanger Sequencing; Clinical Sample Validation; ROC Analysis; Survival Analysis; Transfection; Wound Healing Assay; Transwell Assay; CCK8; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Co-IP; Western Blot; IF (Immunofluorescence); IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
upregulated in CRC with liver metastasis; AUC 0.8433 for predicting CRC liver metastasis; high circ-YAP associated with shorter survival time |
| Description: |
circ-YAP is upregulated in CRC with liver metastasis and predicts worse survival. It promotes migration/invasion and liver metastasis by m6A-dependent translation into YAP-220aa, which binds LATS1 to activate YAP nuclear signaling, and YAP/TEAD further enhances circ-YAP transcription to form a positive feedback loop. |
| Confidence score: |
0.9003 |