| Expression pattern: |
DN |
| Associated gene: |
TUSC2, METTL3 |
| Associated microRNA: |
miR-661 |
| Biological function: |
suppresses proliferation, migration, and invasion of colorectal cancer cells |
| Molecular mechanism: |
acts as a competing endogenous RNA (ceRNA) sponging miR-661 to upregulate TUSC2; METTL3-mediated m6A methylation enhances circ_RUSC2 stability |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (promotes); m6A modification (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry); Bioinformatics Analysis; MeRIP / MeRIP-seq; Clinical Sample Validation |
| Clinical significance: |
circ_RUSC2 downregulation correlates with advanced stage, lymphatic and distant metastases, and poor differentiation |
| Description: |
circ_RUSC2 is downregulated in colorectal cancer tissues and cell lines and is associated with more aggressive clinicopathological features. Functionally, circ_RUSC2 suppresses CRC cell proliferation, migration and invasion by sponging miR-661 to relieve repression of the tumor suppressor TUSC2. Its stability is enhanced by METTL3-associated m6A modification. |
| Confidence score: |
0.8432 |