| Expression pattern: |
DN |
| Associated gene: |
RBM3, uXBP1 mRNA, XBP1, sXBP1, CST6, DNAJB11, PDIA6, NOVA-1 |
| Associated microRNA: |
- |
| Biological function: |
Inhibits invasion, migration, lymphangiogenesis, and lymphatic metastasis in nasopharyngeal carcinoma. |
| Molecular mechanism: |
circFAM13B binds the RBP RBM3 and enhances RBM3-uXBP1 mRNA interaction, promoting uXBP1 mRNA degradation, reducing sXBP1 and CST6 transcription/secretion, thereby suppressing ER stress-related pro-metastatic signaling and lymphangiogenesis. |
| Biological pathway or process: |
ERK (not specified); EMT (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); angiogenesis (inhibits); inflammation (not specified); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; FISH / smFISH; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Actinomycin D / DRB Stability Assay; Transfection; Transwell Assay; Wound Healing Assay; CCK8; Colony Formation Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); Luciferase Reporter Assay; ChIP / ChIP-seq; ELISA; Western Blot; Flow Cytometry（Non-apoptosis/cycle）; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circFAM13B expression was associated with better prognosis in NPC and was an independent prognostic factor for progression-free survival. |
| Description: |
circFAM13B is downregulated in lymph node-metastatic nasopharyngeal carcinoma and functions as a tumor suppressor. It localizes to the ER and binds RBM3 to enhance RBM3-mediated uXBP1 mRNA decay, reducing sXBP1 activity and CST6 expression/secretion, thereby inhibiting lymphangiogenesis and lymphatic metastasis. High circFAM13B expression is associated with better patient prognosis. |
| Confidence score: |
0.8941 |