circRNA basic information
circBase ID: -
Name: hsa_circ_CDR1AS
Synonym: CircCDR1as
Host Gene: -
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0004958
MONDO name: oral cavity squamous cell carcinoma
Disease details: oral squamous cell carcinoma
Disease DO ID:
0050866
Disease MeSH ID:
-
Disease NCIt ID:
C4833
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

OSCC tissues; adjacent normal tissues; tumor tissue sections

Cell lines:

Tca-8113; SCC-15; HOK

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: AKT, ERK½, mTOR, TFEB, LAMP2, ROS
Associated microRNA: miR-671-5p, hsa-miR-7, hsa-miR-1270
Biological function: promotes autophagy; promotes cell survival/viability and proliferation; inhibits apoptosis; promotes tumor growth under hypoxia; promotes ER stress
Molecular mechanism: Acts as a ceRNA/miRNA sponge for miR-671-5p to promote autophagy; enhances hypoxia-induced autophagy by activating AKT and ERK½ and inhibiting mTOR activity; increases lysosomal activity regulators (TFEB, LAMP2) and ROS under hypoxia
Biological pathway or process:

autophagy (promotes); apoptosis (inhibits); proliferation (promotes); ERK (promotes); PI3K/AKT (promotes); PI3K/AKT/mTOR (inhibits); other pathway/process (promotes); ceRNA regulation (promotes)

Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; Luciferase Reporter Assay; Western Blot; IF (Immunofluorescence); CCK8; Annexin V/PI Flow Cytometry; Bioinformatics Analysis; IHC (Immunohistochemistry); In Vivo Animal Model

Clinical significance:

patients with a higher circCDR1as expression had shorter postoperative survival time than those with lower circCDR1as expression

Description:

CircCDR1as is up-regulated in OSCC tissues/cell lines and further induced by hypoxia. It promotes OSCC cell survival and tumor growth by enhancing autophagy under hypoxia via AKT/ERK activation and mTOR inhibition, and acts as a sponge for miR-671-5p. High circCDR1as expression is associated with shorter postoperative survival.

Confidence score:

0.7408

Other information
Title:

CircCDR1as upregulates autophagy under hypoxia to promote tumor cell survival via AKT/ERK½/mTOR signaling pathways in oral squamous cell carcinomas.

Journal: Cell death & disease
Published: 2019
PubMed ID: 31582727
Study type:

combined biological and clinical study

Data availability: Supplementary Information accompanies this paper at (10.1038/s41419-019-1971-9).
Code availability: -