circRNA basic information
circBase ID: hsa_circ_0001955
Name: hsa_circ_CSNK1G1
Synonym: hsa_circ_101555
Host Gene: CSNK1G1
Genomic location(hg19): chr15:64495280-64508912:-
Genomic location(hg38): chr15:64203081-64216713:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; corresponding normal-appearing tissues; tumor tissues; normal colon tissues

Cell lines:

SW1116; SW620; HCT116; DLD-1; KM12; HT29; LOVO; SW480; HEK-293T

In vivo animal model:

cell line-derived xenograft; other disease animal model

circRNA-disease information
Expression pattern:
UP
Associated gene: CDK6, RPA3
Associated microRNA: miR-597-5p, miR-145-5p, miR-30a-3p
Biological function: circ101555 promotes CRC cell proliferation, cell cycle progression, colony formation, DNA repair capacity and tumorigenesis, while inhibiting apoptosis; circ101555 knockdown suppresses these oncogenic phenotypes and induces apoptosis.
Molecular mechanism: circ101555 functions as a competing endogenous RNA and sponge of miR-597-5p, thereby up-regulating CDK6 and RPA3 expression in CRC.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (other); other pathway/process (promotes)

Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; IF (Immunofluorescence); Western Blot; IHC (Immunohistochemistry); TUNEL; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

High circ101555 expression is associated with larger tumor size, lymph node metastases, advanced stage, distant metastases, worse 5-year overall survival and disease-free survival; circ101555 may serve as a prognostic indicator in CRC.

Description:

circ101555 is up-regulated in CRC tissues and acts as an oncogenic circRNA. It promotes CRC progression by sponging miR-597-5p and increasing CDK6 and RPA3 expression, thereby enhancing proliferation, cell cycle progression, DNA repair and tumorigenesis while suppressing apoptosis. High circ101555 expression predicts poorer OS and DFS, supporting its potential as a prognostic biomarker and therapeutic target.

Confidence score:

0.8942

Other information
Title:

Hsa_circ_101555 functions as a competing endogenous RNA of miR-597-5p to promote colorectal cancer progression.

Journal: Oncogene
Published: 2019
PubMed ID: 31300733
Study type:

combined biological and clinical study

Data availability: GSE126095; http://www.circbase.org; https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE126095
Code availability: -