| Expression pattern: |
UP |
| Associated gene: |
GLI2, ELAVL1, PRDM1, ETFA promoter |
| Associated microRNA: |
miR-579-3p |
| Biological function: |
promotes proliferation; inhibits G2/M phase arrest (cell cycle arrest) |
| Molecular mechanism: |
PRDM1 transcriptionally activates circ_0036412; circ_0036412 acts as a ceRNA to sponge miR-579-3p and up-regulate GLI2; circ_0036412 recruits ELAVL1 to stabilize GLI2; activates Hedgehog signaling |
| Biological pathway or process: |
Hedgehog (promotes); proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; divergent primers PCR; CCK8; EdU Staining; TUNEL; Cell Cycle Assay; FISH / smFISH; Luciferase Reporter Assay; ChIP / ChIP-seq; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Transfection; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0036412 is up-regulated in HCC cell lines and promotes proliferation while preventing G2/M cell-cycle arrest. Mechanistically, it activates Hedgehog signaling by increasing GLI2 through miR-579-3p sponging and by recruiting ELAVL1 to stabilize GLI2 mRNA; PRDM1 transcriptionally activates circ_0036412 via the ETFA promoter. |
| Confidence score: |
0.808 |