| Expression pattern: |
UP |
| Associated gene: |
PTEN, Akt, mTOR, p-Akt, p-mTOR |
| Associated microRNA: |
- |
| Biological function: |
Promotes CRC cell proliferation, invasion, growth, metastasis and disease progression; knockdown inhibits growth and metastasis abilities. |
| Molecular mechanism: |
Circ-0104631 promotes CRC cell growth and metastasis by inhibiting PTEN expression and promoting the AKT/mTOR pathway; Circ-0104631 knockdown increases PTEN protein and decreases p-Akt and p-mTOR. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Survival Analysis |
| Clinical significance: |
High expression was associated with high TNM stage, distant metastasis and poor prognosis; Circ-0104631 could be used as a prognostic marker for CRC. |
| Description: |
Circ-0104631 is up-regulated in colorectal cancer tissues and CRC cell lines, and its high expression is associated with advanced TNM stage, distant metastasis, and poor patient survival. Functionally, Circ-0104631 promotes CRC cell proliferation, invasion, growth and metastasis, likely through suppression of PTEN and activation of the AKT/mTOR signaling pathway. The study proposes Circ-0104631 as a potential prognostic marker and therapeutic target in CRC. |
| Confidence score: |
0.6194 |